Keywords
Argentina
diarrhea
G8P[8]
G9P[4]
How to Cite
Abstract
Rotavirus A (RVA) presents common and unusual G-/P- associations. The re-assortment between strains during a co-infection is one of the most important mechanisms in generating genetic diversity. In Argentina, two unusual associations (G9P[4] and G8P[8]) were detected for the first time during 2017-2018. It is unknown whether these strains represent new introductions or are the result of reassortment between local common associations. Objective. To analyze the phylogenetic and evolutionary relationships of the G9P[4] and G8P[8] strains respect to the common associations that circulated during the same period. Materials and Methods. Representative RVA strains of both common (n=11) and unusual associations (n=2) detected during 2016-2018 were included in this study, as part of national epidemiological surveillance. Nucleic acids were extracted from fecal samples, and all 11 genomic segments were amplified and sequenced. The genetic constellations of all strains were determined, phylogenetic trees were constructed, and nucleotide and amino acid distance matrices were generated. Results. The constellations of both associations were G9P[4]-I2-R2-C2-M2-A2-N2-T2-E6-H2 and G8P[8]-I2-R2- C2-M2-A2-N2-T2-E2-H2. In 10 segments, the G8P[8] strain was more closely related to a G3P[8] strain that circulated in 2016. In 6 genes, G9P[4] was more closely related to a G2P[4] strain that circulated in 2016, whereas in 2 genes it was more closely related to a G3P[8] strain from 2016, and in 1 gene to a G2P[4] strain from 2017. However, no reassortment events were observed in any of the 11 segments. Conclusions. The emerging G9P[4] and G8P[8] strains detected in Argentina are the result of novel introductions into our country.
References
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